Coding
Part:BBa_K2272014:Design
Designed by: Eric Joshua Wang Group: iGEM17_Virginia (2017-10-22)
pBAD-Cytochrome(Cyt)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal XhoI site found at 1189 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 171
Illegal NgoMIV site found at 219
Illegal NgoMIV site found at 225
Illegal NgoMIV site found at 240
Illegal NgoMIV site found at 338
Illegal NgoMIV site found at 386
Illegal NgoMIV site found at 392 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1788
Illegal BsaI.rc site found at 1944
Design Notes
The araC-pBAD promoter and the cytochrome coding sequence was ligated with SCAR.
Source
Distribution kit for araC-pBAD.