Coding

Part:BBa_K2272014:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pBAD-Cytochrome(Cyt)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 1189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 171
    Illegal NgoMIV site found at 219
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 240
    Illegal NgoMIV site found at 338
    Illegal NgoMIV site found at 386
    Illegal NgoMIV site found at 392
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1788
    Illegal BsaI.rc site found at 1944


Design Notes

The araC-pBAD promoter and the cytochrome coding sequence was ligated with SCAR.


Source

Distribution kit for araC-pBAD.

References